Analysis of the Interaction Between Bicine buffer 150-25-4 and Metal Ions

The structural characteristics and coordination basis of Bicine

Metal elements in living organisms mostly exist in the form of complexes, often binding to biomolecule ligands such as proteins and nucleic acids, and the basic building blocks of proteins are amino acids. The N, N-di (2-hydroxyethyl) glycine (Bicine buffer) synthesized by KiplranoV for the first time in 1926, as an analog of glycine, possesses dual properties of amino acid and amino ethanol. Structurally, Bicine contains substituted amino groups, one carboxyl group, and two hydroxyl groups, all of which possess coordination ability, enabling it to form complexes with metal ions. The amino acid structure is the core site for its bonding with metal ions.

The characteristic advantages of Bicine as a buffering agent

In 1966, Good et al. found that Bicine had a suitable pH range of 7.6-9.0, which was very suitable for the hydrogen ion buffering needs of biological systems, and was therefore classified as a "Good's" buffer. This buffer has multiple characteristics suitable for biological research: it is soluble in water but insoluble in organic solvents such as dimethylformamide (DMF), dimethyl sulfoxide (DMSO), and dimethylacetamide (DMAc); A 25% concentration aqueous solution appears colorless and transparent; The weak salt effect and difficulty in penetrating biological membranes make it widely used in the field of biological research.

Bicine powder

Cognition of the interaction between Bicine and metal ions

Previous studies have hypothesized that Bicine, as a buffering agent, has only weak or no interaction with metal ions, but this hypothesis has been proven to be unreasonable. In fact, buffering agents may interact with metal ions by forming complexes, which can affect the effectiveness of some research results due to the concentration of buffering agents. For example, when calculating the binding constant between proteins and metal ions, ignoring the interaction between buffering agents and metal ions can lead to erroneous conclusions. Numerous experiments have confirmed that Bicine can form stable binary and ternary complexes with metal ions, and researchers have conducted extensive exploration on the stability of these complexes in solution.

The research significance and application tips of interactions

The interaction between Bicine and metal ions has gradually received attention from the research community. Due to the weaker coordination groups of Bicine's two hydroxyl groups, mixed coordination complexes are easily formed when other ligands with stronger coordination abilities are present in the system. This characteristic suggests that caution should be exercised when using Bicine as a buffer in systems containing metal ions and potentially coordinating biological ligands. The metabolic reactions in organisms involve the balance between various metal ions and donor molecules, and studying the coordination balance between transition metal ions and various ligands in vitro can provide accurate references for analyzing coordination phenomena in organisms. Therefore, there is still ample room for further research on the interaction between Bicine, as an amino acid analogue, and transition metal complexes.

Product packaging

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