Lactate dehydrogenase IVD diagnostic reagent: an important tool in the diagnosis of myocardial infarction

In the field of in vitro diagnostics (IVD), biochemical diagnostic reagents are the key support for disease diagnosis, and lactate dehydrogenase (LDH) IVD diagnostic reagents are a typical representative. It relies on the biochemical characteristics of LDH to provide accurate detection basis for the diagnosis of cardiovascular diseases such as myocardial infarction. This article analyzes its technical principles, application value, and clinical precautions from the perspective of IVD.

1, Technical principle and performance indicators of lactate dehydrogenase IVD diagnostic reagent

The detection principle of LDH IVD diagnostic reagent is based on the specific reaction catalyzed by LDH: with the participation of coenzyme, LDH catalyzes the reversible conversion of pyruvate and lactate, accompanied by the conversion of NADH and NAD ⁺, and NADH has characteristic absorbance at specific wavelengths. The reagent can calculate the LDH activity concentration in the sample by detecting the rate of NADH absorbance change, achieving quantitative detection.

Lactate Dehydrogenase

High quality reagents need to meet clinical needs: the detection range covers the normal range of serum LDH (135-215U/L) and the range of pathological elevation; Having good specificity and avoiding interference from other enzymes, hemolytic substances, etc; At the same time, it is necessary to have a stable refrigeration shelf life, room temperature stability, and batch consistency to ensure the repeatability of testing.

2, The application value of lactate dehydrogenase IVD diagnostic reagent in the diagnosis of myocardial infarction

When myocardial infarction occurs, coronary artery occlusion causes myocardial cell necrosis, and intracellular LDH is released into the bloodstream, resulting in an increase in serum LDH. This change needs to be captured by LDH IVD diagnostic reagents to provide data support for diagnosis.

Its application value is reflected in two aspects: firstly, it can supplement the diagnosis in the later stage. Compared with early markers such as troponin (with a shorter window period), LDH has a longer period of elevation, which can fill the diagnostic gap of patients who have been ill for more than 24 hours; The second is the improvement of diagnostic specificity. LDH isoenzymes IVD diagnostic reagents can detect myocardial specific LDH1. If the LDH1/LDH2 ratio is greater than 1, it can accurately point to myocardial injury and reduce the risk of misdiagnosis.

In addition, LDH diagnostic reagents are compatible with fully automatic biochemical analyzers, and batch processing of samples takes short time, meeting the urgent need for "fast results" and buying time for rescue.

3, Precautions for clinical application of lactate dehydrogenase IVD diagnostic reagent

Firstly, standardize sample processing. LDH exists in red blood cells, and sample hemolysis can cause false elevation of test results. It is necessary to collect and remove hemolysis samples in a standardized manner, and the samples should be promptly centrifuged to separate serum to prevent a decrease in LDH activity.

Secondly, follow the reagent usage process. The buffer formula and incubation temperature of different brands of reagents may vary, so please follow the instructions and refrigerate the reagents to avoid repeated freezing and thawing.

Finally, provide a comprehensive interpretation of the results. LDH is not a specific biomarker for myocardial infarction, and it can also be elevated by liver diseases. It needs to be comprehensively judged based on the patient's symptoms, electrocardiogram, and troponin test results to ensure accurate diagnosis.

In summary, LDH IVD diagnostic reagents provide key support for the diagnosis of myocardial infarction. Standardized operation and comprehensive interpretation in clinical application are necessary to fully realize their value and assist in accurate diagnosis and treatment.

Company Realistic Picture

Hubei Xindesheng Material Technology Co., Ltd. has always adhered to the production and operation philosophy of "quality first, technology leadership" and "innovation and pursuit of excellence", continuously researching and developing reagents needed in the diagnostic field. Since its establishment in 2005, it has a history of nearly 20 years and is deeply trusted and supported by users. The diagnostic enzyme reagents independently developed and produced by Desheng have been sold to various parts of the world. If you have any related procurement needs in the near future, please feel free to contact us!